Cannabis Cultivation and Science Podcast
- Folgen
- 170
- Erscheint
- monatlich
- Letzte Folge
- 12. Nov. 2026
Über den Podcast
Welcome to the Cannabis Cultivation and Science podcast, I’m your host, Tad Hussey of KIS Organics. This is the podcast where we discuss the cutting edge of organic growing from a science based perspective and draw in top experts from around the industry to share their wisdom and knowledge. If you want to hear the latest in growing technology and methods, this is the place..
Folgen 170
Cannabis Grow Room Controllers and Sensors: From Tents to Greenhouses with Kelley Nicholson
What we cover in this episode:What to monitor in a grow tent or small room, including temperature, humidity and VPD, and why sensor quality matters so much.Why your sensors belong in the plant canopy, not on …
Shownotes
What we cover in this episode:
- What to monitor in a grow tent or small room, including temperature, humidity and VPD, and why sensor quality matters so much.
- Why your sensors belong in the plant canopy, not on the wall or the AC unit, and when you need a second sensor or a sensor on each tier.
- Scaling up to greenhouses: how many sensors you need, why greenhouse length matters more than width, and how zones are set up.
- Controller tiers and costs, from Trolmaster-style systems for small grows up to larger systems like Autogrow and Wadsworth, plus what 0–10 volt control does for lighting and fans.
- Common mistakes: outgrowing an inexpensive controller, equipment that won't restart on its own after a power cycle, and planning for labor as you scale.
- Switching controller brands, training and support, and getting through your first year of seasonal swings in a greenhouse.
- Remote access and historical data, like spotting nighttime humidity buildup before it turns into a problem.
- Automated irrigation, fertigation and moisture probes, including a Humboldt facility running four cultivars as four irrigation zones.
Black Friday Sale, November 10–30: 25% off select KiS Organics products plus three Black Friday bundles. No code needed. Shop the sale.
Show notes and related products: kisorganics.com
Learn more about Autogrow: autogrow.comIf you got something out of this episode, hit follow in your podcast app so you don't miss the next one. Happy Growing!
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Cannabis Aroma Science Beyond THC: Volatile Compounds and Chemotaxonomy with Dr. Riley Kirk & Nature’s Heritage
What we cover in this episode:The Fallacy of THC Percentage: Why consumer purchasing metrics fail to predict actual experience and how aroma offers a more reliable sensory guide.The Six Aroma Categorizations …
Shownotes
What we cover in this episode:
- The Fallacy of THC Percentage: Why consumer purchasing metrics fail to predict actual experience and how aroma offers a more reliable sensory guide.
- The Six Aroma Categorizations: How MariMed and Dr. Kirk broke down complex strain chemistry into six practical sensory buckets—from Jacks & Haze to OG, Gas & Chem.
- Analytical COA Limitations: Why standard terpene panels miss low-abundance, high-potency molecules like volatile sulfur compounds (VSCs) and flavorant esters that dominate a strain's scent profile.
- Cultivation & Post-Harvest Precision: Kris Roy breaks down how drying and curing environments, water activity target thresholds, and operational SOPs preserve volatile monoterpenes and sesquiterpenes.
- Combustion vs. Volatilization Chemistry: What happens to monoterpenes versus sesquiterpenes when flower is lit at high heat, and why joint dynamics shift from head-heavy to body-heavy notes as you smoke.
- Industry Nuance & MSOs: A candid discussion on craft cultivation, scale, consumer safety, and the role of large-scale operators in funding plant science research.
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Growing in Living Soil and Passing Heavy Metals Testing with Greg Larson
How do you maintain a high-fertility organic living soil program in states with near-zero tolerance for heavy metals?In this episode, Tad Hussey (KISS Organics) sits down with Greg Larson, a commercial cannabis …
Shownotes
How do you maintain a high-fertility organic living soil program in states with near-zero tolerance for heavy metals?
In this episode, Tad Hussey (KISS Organics) sits down with Greg Larson, a commercial cannabis consultant specializing in organic soil systems and heavy metal mitigation. Together, they break down the science and practical management of heavy metals in commercial cannabis cultivation, drawing on real-world data from facilities operating under tight compliance limits in South Dakota and Minnesota.
What You'll Learn in This Episode:
- Root Cause Testing: How to use ICP-MS lab testing to audit every facility input—from soil amendments and fertilizers down to irrigation lines and trim gloves.
- Input Selection Dynamics: Why ocean-based inputs (like fishbone meal) carry significantly higher heavy metal risks compared to land-based options (like bovine bone meal, feather meal, and soybean meal).
- Soil Chemistry & Plant Pathways: How maintaining optimal phosphorus levels and neutral pH prevents plants from absorbing arsenic through shared uptake mechanisms.
- Biomass Removal Strategies: Why pulling root balls instead of "chopping and dropping" removes accumulated heavy metals and systematically lowers soil contamination cycle after cycle.
- Beds vs. Containers: How larger soil volumes act as a buffer against heavy metal spikes while lowering labor costs and nitrogen requirements.
- Irrigation Fail Points: Managing reverse osmosis (RO) systems, preventing metal stripping from plumbing, and avoiding anaerobic soil pockets that increase arsenic bioavailability.
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Soil Microbes for Growing Cannabis: Dr. Colin Bell on Microbial Consortia, Rhizosphere Ecology and Mammoth P
Key Takeaways & Highlights Mammoth P Relaunch & Product Updates The Return: After stepping away in late 2022 and spending three years working with the Microbial Mass Pro team, Colin bought back and officially …
Shownotes
Key Takeaways & Highlights
Mammoth P Relaunch & Product Updates
The Return: After stepping away in late 2022 and spending three years working with the Microbial Mass Pro team, Colin bought back and officially relaunched the Mammoth brand.
~70% Price Reduction: Streamlined, scalable manufacturing allowed Colin to drop the price by roughly 70% compared to legacy pricing, making it significantly more accessible for all growers.
3-Year Shelf Life: A subtle reformulation extends the active, non-spore liquid shelf life out to three full years.
Application Rate: Continuous weekly applications at 0.6 mL per gallon throughout veg and bloom yield the best results across all substrates (living soil, hydro, coco, and rockwool).
Mammoth P vs. Microbial Mass Pro
Mammoth P: Uses active (motile), non-spore-forming bacteria selected directly from natural soil samples to liberate phosphorus and trace metals like iron. It is carried in an organic, alfalfa-derived liquid medium.
Microbial Mass Pro: Uses dormant Bacillus endospores suspended in a clean, synthetic-like solution. While Bacillus spores require 24–48 hours to germinate once applied, active motile strains start functioning immediately upon contact with the root zone.
The Power of Consortia
Microbes in nature do not work in isolation. Multi-species consortia offer synergistic functionality (1+1+1+1 = 5, 6, or 7) and greater ecological adaptability across changing pH, moisture levels, and soil micro-pores than single-species products.
Addressing Common Grower Myths
Brewing into Compost Tea: Discouraged. Tank brewing introduces unpredictable competitive dynamics, risks losing the intended consortia balance, and isn't necessary given the product's concentration and price point.
The Triacontanol Myth: Alfalfa meal is used strictly as a carbon and nitrogen source to feed the bacteria, undergoing heavy boiling and filtration to sterilize the media. Independent testing confirmed triacontanol levels were untraceable or present in negligible parts-per-billion amounts—it is not the driver of performance.
Pathogen Concerns: Colin debunked rumors regarding human pathogens, explaining strain specificity (the same way safe E. coli strains differ from harmful ones) and highlighting full regulatory approval across international agricultural departments.
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Why Cannabis THC Test Results Vary: Lab Potency Testing with Cannabis ChemLab
This episode covers the key scientific drivers behind lab result variability, from instrument calibration down to field sampling practices. Understanding Reference Standards: How analytical instruments use …
Shownotes
This episode covers the key scientific drivers behind lab result variability, from instrument calibration down to field sampling practices.
Understanding Reference Standards: How analytical instruments use commercially available reference standards to construct calibration curves for testing unknown samples.
The Potency Variability Study: Insights from Cannabis ChemLab’s study overlaying 35 different THCA standards, demonstrating how variance between standard manufacturers and product lots leads to drastic differences in reported THC percentages.
The Mechanism of Potency Inflation: How calibration curves at the high end of concentration explain "impossible" lab results exceeding 100% cannabinoids in concentrates and diamonds.
Neutral vs. Acidic Cannabinoids: Why acidic forms like THCA and CBDA present significantly more analytical variability in the lab compared to decarboxylated neutral forms found in edibles.
Heavy Metals and Fertilizer Inputs: An in-depth discussion on ICP-MS heavy metal testing, identifying soil amendments like fishbone meal as a primary source of arsenic accumulation in living soil.
Dispelling Marketing Claims: Why ocean-based inputs are not inherently cleaner than other fertilizers, and why growers must screen raw materials rather than relying on marketing claims.
Agronomic Mitigation Strategies: How plant biology and strategic tissue management, such as utilizing older lower leaves as heavy metal sinks, can help keep heavy metals out of the final flower.
Navigating Unexpected Results: Practical steps for cultivators when faced with surprising lab data, including when a flower batch warrants resampling versus when a process error has occurred.
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Cannabis Hyperemesis Syndrome (CHS): Is It Really Bad Weed? with Dr. Codi Peterson
The Reality of CHS and the 1,100+ Patient Study Dr. Peterson highlights the scope of his landmark survey, which gathered data from 1,134 CHS sufferers. The study reveals that CHS is not an acute reaction to a …
Shownotes
The Reality of CHS and the 1,100+ Patient StudyDr. Peterson highlights the scope of his landmark survey, which gathered data from 1,134 CHS sufferers. The study reveals that CHS is not an acute reaction to a single session, but rather a chronic, low-level dysregulation of the endocannabinoid system (ECS) that predominantly impacts heavy, long-term consumers.
Frequency of Use: 96.5% of study participants consumed cannabis daily, and 45% consumed more than six times per day.
The "Buoy" Metaphor: Dr. Peterson explains the ECS as a master regulatory buoy. While occasional cannabis use gently nudges the buoy, chronic high-dose THC overstimulation completely knocks it over, triggering the cyclical vomiting state.
Vapes vs. Flower: The Onset TimelineOne of the most significant findings of the paper is how the method of inhalation changes how quickly CHS develops.
Vaporizing: Exclusive vapers reported symptoms starting within 1 to 2 years of beginning use.
Smoking Flower: Flower smokers took significantly longer to develop CHS, with the most common timeline being 10 years or more.
Debunking the Pesticide and Neem Oil MythsA popular theory in the cannabis community is that CHS is actually pesticide or neem oil poisoning. Dr. Peterson challenges this theory using clinical toxicology:
The Sourcing Data: Sufferers in the study reported identical CHS symptoms regardless of whether they got their weed from licensed dispensaries, illicit sources, or their own pesticide-free home grows.
Neem Oil Toxicity: Ingesting neem oil causes immediate nausea, alongside severe clinical signs like liver damage, seizures, and brain swelling—none of which are observed in CHS patients.
Pesticide Toxidromes: Known pesticide exposures (like organophosphates) present with highly specific chemical "toxidromes" that look nothing like cyclical vomiting.
Gender Differences in Symptom SeverityThe study revealed a stark contrast in how CHS manifests between sexes:
Men: Frequently reported shorter hyperemetic episodes, often lasting only 1 day.
Women: Suffered statistically longer and more severe episodes, with the most common duration being 3 to 5 days, and over 10% suffering for 10 or more days of continuous vomiting.
Recognizing the Prodrome and Navigating RecoveryThe Prodrome Phase: This early warning phase involves morning nausea, abdominal pain, heartburn, and hot/cold flashes. It can linger in the background for years before a full vomiting episode occurs.
The Path to Healing: While the clinical textbook demands absolute abstinence, Dr. Peterson shares real-world data showing how patients actually navigate recovery, the limitations of hot baths as temporary relief, and the potential future role of minor cannabinoids like CBG for harm reduction.
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Why Cannabis Clones Lose Vigor Over Time: Genetic Drift in Propagation
The Mechanics of Genetic Drift Plants are natural genetic mosaics. As they grow and divide over time, typos naturally accumulate in their DNA code, which are known as somatic mutations. Traditional vegetative …
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The Mechanics of Genetic Drift Plants are natural genetic mosaics. As they grow and divide over time, typos naturally accumulate in their DNA code, which are known as somatic mutations. Traditional vegetative cuttings and field-grown plants accumulate these mutations at a very low, linear baseline rate over decades. However, the extreme genomic degradation highlighted in the paper is driven by rapid generational cell cycling in vitro. Forcing an unorganized mass of cells to constantly divide and regenerate bypasses the plant's normal cellular quality control checkpoints.
Somatic Embryos vs. Meristem Cultures Somatic embryogenesis involves stripping a plant down to a single cell or callus phase to regenerate an embryo. This is considered high risk for mutations because if the founder cell contains a mutation, that mutation becomes permanently fixed in every single cell of the thousands of plants propagated from it. This differs from meristem tip culture, which is the low risk, predominant form of tissue culture used in cannabis for clearing Hop Latent Viroid. Because meristem tip culture preserves the existing, highly organized architecture of the shoot apical meristem rather than forcing single cells to reprogram, the genetic risk is fundamentally lower.
DNA Typos vs. Chromosome Catastrophes The high-stress cell cycling environment caused macroscopic genomic errors, such as whole-chromosome duplications called trisomies. In the walnut clones, these large-scale errors made it incredibly difficult to grow the tissue back out into healthy adult plants. The stress of rapid lab cell cycling also desilenced transposable elements, or jumping genes, allowing pieces of DNA to insert themselves randomly throughout the genome and disrupt normal gene expression. It is worth noting that not all mutations are bad. Small point mutations, or single base changes, happen constantly in nature as life being life. Most occur in non-coding regions and have zero impact, while a rare few can actually create desirable new traits as seen historically in citrus and wine grapes.
Genetics vs. Epigenetics The guests distinguish between true genetic mutations, which are permanent changes to the A, C, T, G code, and epigenetic modifications, which act like chemical bookmarks such as methylation that change how a gene is read without altering the underlying sequence. Some instances of a mother plant losing vigor may actually be epigenetic shifts caused by environmental stress or endophyte and pathogen buildup over time, rather than a permanent genetic mutation.
Actionable Advice for Cannabis Nurseries Commercial operations scaling up clones to the tens of thousands should utilize genetic sequencing early in the pipeline. Testing the early founder material can easily catch large-scale catastrophic mutations like chromosome duplications or deletions, protecting the nursery from mass-producing defective production plants. Whole-genome sequencing costs have plummeted significantly, and the guests encourage cultivators and nursery operators with unique, degenerating, or shifting clonal lines to collaborate with academic labs to sequence the data and map exactly what is changing.
Guest Information and Contact Details
J. Grey Monroe is an Associate Professor in the Department of Plant Sciences and the Genome Center at UC Davis. His lab studies how mutations arise across genomes, DNA repair, epigenomics, and genome evolution in plants, with applications in crop improvement and protection. Lab website: https://monroelab.org/ Email: [email protected]
Matthew Davis is a fifth year PhD candidate in the Plant Biology Graduate Group co-advised by Grey Monroe and Pat J. Brown. His research focuses on somatic mutation in orchard crops, an understudied genetic process that directly affects one of California’s largest agricultural industries. Bluesky: @davismw.bsky.social Email: [email protected]
Research Links and Resources
Peer-Reviewed Paper: https://www.pnas.org/doi/10.1073/pnas.2530182123 Shorter general audience article: https://www.ucdavis.edu/blog/not-all-clones-are-created-equal Slightly longer general audience article: https://www.plantsciences.ucdavis.edu/news/davis-monroe-somatic-mutations
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Cannabis Growing Myths Debunked with Dr. Deron Caplan
In this conversation, host Tad Hussey (KIS Organics) and Dr. Deron Caplan (CannaCribs Horticulture Consulting) discuss the intersection of agricultural science and commercial cannabis cultivation, focusing …
Shownotes
In this conversation, host Tad Hussey (KIS Organics) and Dr. Deron Caplan (CannaCribs Horticulture Consulting) discuss the intersection of agricultural science and commercial cannabis cultivation, focusing heavily on irrigation, lighting, and research methodologies.
Drought Stress vs. Drybacks: Dr. Caplan clarifies a major industry misconception by distinguishing standard irrigation "drybacks" from actual "drought stress." He explains that drybacks work primarily by pulling oxygen into the root zone (which cannabis loves), whereas true drought stress requires pushing the plant past the point of water availability.
The Nuance of Cannabinoid Bumps: While Dr. Caplan’s landmark PhD research found that controlled drought stress in week seven of flowering could boost THC/CBD content by 30% to 40% without losing yield, he notes that repeating this in smaller pots with faster drybacks yielded no positive results. This highlights just how incredibly nuanced and difficult it is to trigger beneficial plant stress without harming the crop.
Organics vs. Mineral Salts at Scale: The two debate the logistics of commercial cultivation. While mineral salts offer strict baseline consistency and easier pathogen sterilization for medical export markets, Dr. Caplan notes that data-driven, evidence-based living soil systems have come a long way and are proving to be increasingly scalable.
Debunking the Leaf-Tip Cloning Myth: Dr. Caplan shares that his early research on propagation disproved a ubiquitous legacy market myth: cutting the tips off clone leaves actually reduces rooting success, unless leaves are so large that they are actively shading adjacent clones.
Advanced Canopy Management: They discuss the massive yield and quality benefits (15% to 20% increases) of under-canopy lighting (UCL). Dr. Caplan details how commercial facilities can use a PAR meter to calculate the Leaf Area Index (LAI) to mathematically standardize how much foliage to prune, rather than relying on visual guesswork.
The Future of Research: Dr. Caplan highlights his new commercial R&D facility in Canada designed to test applied science practices. They close by discussing the validity of peer-reviewed data versus private white papers, concluding that regardless of a grower's background, scientific data is the ultimate tool for bridging the gap between organic and conventional cultivation.
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Can a 13-Hour Light Cycle Boost Cannabis Yields 50%? with Dr. Youbin Zheng
In this episode, we discuss: Bridging the Genetic Gap: Looking critically at how horticultural research transfers between high-THC cultivars and industrial hemp. The 13-Hour Photoperiod: Breaking the …
Shownotes
In this episode, we discuss:
Bridging the Genetic Gap: Looking critically at how horticultural research transfers between high-THC cultivars and industrial hemp.
The 13-Hour Photoperiod: Breaking the traditional 12/12 cycle to achieve a 39%–50% yield increase and a 9% THC boost.
Linear Light Scaling: The direct relationship between light intensity and flower yield scaling all the way up to 1,800 micromoles under ambient CO2.
The UV Reality Check: Why modern high-THC genetics actually showed a decrease in final cannabinoid and terpene content under supplemental UVA and UVB.
Light Response Curves: Why relying on a single leaf measurement to guide your facility's light saturation point is fundamentally flawed.
The Veg-to-Flower Transition: Practical SOPs for adjusting PPFD and DLI safely without shocking your canopy.
Controlled Deficit Irrigation: How a single, targeted late-flower drought stress event triggers a 12%–13% spike in final THC and CBD content.
The 60 PPM Phosphorus Rule: Looking at the established replication data proving that excess phosphorus wastes money, reduces yield efficiency, and impacts the environment.
Links & Resources Mentioned in This Episode:
Visit KIS Organics for commercial living soils, amendments, and consulting: https://www.kisorganics.com
Grab Dr. Zheng's textbook, Handbook of Cannabis Production in Controlled Environments: https://www.amazon.com/Handbook-Cannabis-Production-Controlled-Environments/dp/0367712571
Access Dr. Zheng’s open-access research papers on Google Scholar: https://scholar.google.com/citations?user=ciGdnWAAAAAJ&hl=en
Connect with us on Instagram: @kisorganics
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Cannabis Plant Diseases, Mycotoxins and Microclimates with Dr. Nicole Gauthier
o keep the audience engaged while highlighting the technical value of the episode, this version focuses on the "why" and the high-level takeaways, leaving the "how" for the audio. Podcast Summary In this …
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o keep the audience engaged while highlighting the technical value of the episode, this version focuses on the "why" and the high-level takeaways, leaving the "how" for the audio.
Podcast SummaryIn this episode, Tad Hussey of KIS Organics talks with plant pathologist Dr. Nicole Gauthier to separate fact from fiction regarding cannabis pathogens. The discussion moves past the basics of "mold" to look at the specific science of Fusarium, Botrytis, and the reality of mycotoxins in the supply chain. From the limitations of soil testing to the importance of bud microclimates, Dr. Gauthier provides a professional framework for understanding how plant diseases actually function and how to manage them without relying on "curative" myths.
Key TakeawaysThe Mycotoxin Reality: Why species identification matters more than ever, and a look at which common pathogens actually pose a toxic risk to consumers.
Microclimates vs. Room Environment: Understanding why your sensors might say your room is safe while your "chunky buds" are harboring high-humidity infections.
The Problem with Broad Testing: A critical look at why generic soil tests and "Total Yeast and Mold" plates often provide misleading or incomplete data for growers.
Biological Controls & SAR: The science of "priming" a plant’s immune system and the differing persistence levels of Bacillus and Trichoderma on the leaf surface.
The Pathology "Lightning Round": Dr. Gauthier’s professional advice on sanitation protocols, rogueing diseased plants, and the most effective ways to manage an outbreak.
Featured ExpertDr. Nicole Gauthier is a researcher and extension specialist at the University of Kentucky. Her work focuses on sustainable disease management and helping growers navigate the intersection of plant health and regulatory compliance.
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Concrete Botany: Reconnecting with the Living World with Joey Santore
Key Takeaways & Discussion Highlights The Railroad Roots: Joey’s transition from a Union Pacific engineer to a "vigilante botanist." He discusses the influence of Carl Sagan’s The Demon-Haunted World and using …
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Key Takeaways & Discussion HighlightsThe Railroad Roots: Joey’s transition from a Union Pacific engineer to a "vigilante botanist." He discusses the influence of Carl Sagan’s The Demon-Haunted World and using science as a "candle in the dark" in a consumerist society.
Botany for the "Jaded Working Class": Why academic botany often fails to connect with the public and why Joey targets the "cynical, maladjusted stiff" who needs the living world the most.
Horticultural Atrocities: A critique of "horticultural fluff"—boxwoods, crepe myrtles, and cloned cultivars that offer no ecological value. Joey explains why he’d rather see a lawn than a line of "garbage" cultivars.
The Concrete Cage & Mental Health: How our disconnection from the land and our "unwalkable sprawling excerpts" contribute to a massive mental health crisis.
The Field Record: Joey’s process in the wild—using iNaturalist, herbarium vouchers, and photography to document the "living machine."
Geology & Evolution: An exploration of edaphic endemics (plants that grow only on specific soils like serpentine or gypsum) and how stressful geology creates new species.
Disturbance Ecology: Why "weeds" are actually pioneer species and how disturbance—when understood correctly—is the engine that enables diversity.
Beyond "Is it Edible?": Why the question "Can I eat it?" is the most common and least interesting way to interact with a plant.
Concrete Botany: A preview of Joey’s new book and his fascination with "unintentional ecology" in neglected urban spaces like railroad tracks and abandoned warehouses.
Resources MentionedBook: Concrete Botany: The Ecology of Plants in the Age of Human Disturbance (Released April 2026)
YouTube/Podcast: Crime Pays But Botany Doesn’t
App: iNaturalist (Joey’s primary tool for citizen science)
Literature: The Demon-Haunted World by Carl Sagan
Project: Thornscrub Sanctuary (South Texas conservation)
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Spraying Oils on Cannabis: Dips, Sprays and Avoiding Plant Burn with Julie Graesch
Why can't you just use canola oil from your kitchen to kill spider mites? If you’ve ever wondered about the true science behind oil-based pesticides, this episode is a deep dive into the physics and chemistry …
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Why can't you just use canola oil from your kitchen to kill spider mites? If you’ve ever wondered about the true science behind oil-based pesticides, this episode is a deep dive into the physics and chemistry of plant protection. Tad and Julie Graesch explore why oils remain one of the most effective tools for managing resistant pest populations like two-spotted spider mites and thrips.
In this episode, we cover:
Physical Modes of Action: How oils kill through suffocation and desiccation, and why pests can’t develop resistance to being physically smothered.
Mineral vs. Essential Oils: The functional differences between horticultural mineral oils (like SuffOil-X) and 25B essential oils (like Epishield).
The Power of Formulation: Why inert ingredients, surfactants, and molecular weight are just as important as the active ingredients for preventing phytotoxicity.
Dipping vs. Spraying: The high-stakes world of plant dips and how to target specific life stages—including the elusive thrips egg.
Tank Mixing & Compatibility: Best practices for combining oils with microbials like Beauveria bassiana to create a synergistic knockdown effect.
Whether you are a small-scale hobbyist or a large-scale commercial cultivator, understanding how to master oils will help you maintain a cleaner garden with fewer chemical interventions.
You can order Bioworks products through KiS Organics, just reach out if you have any questions.
About the Guest: Julie Graesch is the Technical Services Manager for BioWorks. With over 18 years of experience as a biological scientist, Julie has worked in laboratory, greenhouse, and field research, specializing in integrated pest management (IPM) for the horticulture industry.
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Botrytis, Bud Rot and Cannabis Pathogens with Dr. Zamir Punja
Key Takeaways for Cultivators The Week 3 Strategy: Preventive sprays for Botrytis must occur when the flowers are still small and "open." Once the bud swells (Weeks 6+), the pathogen is already locked inside …
Shownotes
Key Takeaways for CultivatorsThe Week 3 Strategy: Preventive sprays for Botrytis must occur when the flowers are still small and "open." Once the bud swells (Weeks 6+), the pathogen is already locked inside.
Airflow is Everything: Buds act as heat sinks. Use fans 24/7 to cool the internal temperature of the flower, which is significantly higher than your room’s ambient temperature.
Identify Your Mold: If your bud rot looks pink or white rather than grey, it may be Fusarium. This is a systemic issue that requires a different mitigation strategy and poses a mycotoxin risk.
HLVd Survival: In living soil, HLVd may break down much faster than on sterile surfaces because the microbes decompose the host root tissue.
Watch the Silica: Over-applying potassium silicate can cause the plant to excrete white crystals onto the leaves and stems—don't mistake this "silica vomit" for PM or resin!
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Reading a Soil Test: Agronomy and Crop Health with Lee Briese (Foundations Series #5)
Key Topics & Notes • Why experienced crop consultants rely heavily on chemical soil analysis for making management decisions • The limitations of relying solely on biological soil testing • Why soil structure …
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Key Topics & Notes• Why experienced crop consultants rely heavily on chemical soil analysis for making management decisions
• The limitations of relying solely on biological soil testing
• Why soil structure and aggregation play a critical role in plant health
• How soil physical properties influence water movement, aeration, and root development
• The importance of walking fields and directly interacting with soil
• Using smell, texture, and visual indicators to understand soil conditions
• How agronomists diagnose soil problems using multiple indicators at once
• Why soil management is similar to medical diagnosis — identifying root causes rather than symptoms
• The role of experience and pattern recognition in interpreting soil tests
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What's Your Garden Missing? Applied Horticulture and Limiting Factors with Don Marshall (Foundations Series #4)
Key Takeaways: Botany vs. Hacks: Moving past "quick fixes" to understand the biological lens of plant science. The Limiting Factor: Learning to identify the specific environmental or nutritional gaps holding …
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Key Takeaways:
Botany vs. Hacks: Moving past "quick fixes" to understand the biological lens of plant science.
The Limiting Factor: Learning to identify the specific environmental or nutritional gaps holding your plants back.
Timeless Principles: Why botanical fundamentals are the most reliable tools in a cultivator’s shed, regardless of changing trends.
Applied Horticulture: Bridging the gap between academic theory and high-level landscape maintenance.
About Don Marshall: Don is a Certified Professional Horticulturalist, Certified Arborist, and ecoPro. He founded the Environmental Horticulture program at Lake Washington College, where he taught for over 40 years. He is the owner of Plant Designs, a fine gardening firm, and the co-author of Home Landscaping, Northwest Region.
The Botany Foundations Course: Don and I have spent the last few years collaborating on a comprehensive online botany course. We designed this specifically for industry professionals and serious growers who want a university-level understanding of plant science without the time constraints of traditional schooling. This course provides the professional lens needed to evaluate and improve your grow.
Stay Updated: www.kisorganics.com
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The Soil Food Web Explained: Science, Dogma and Controlled Ecosystems with Tad Hussey (Foundations Series #3)
The Underground Economy: Carbon as Currency Biological Gold: Why photosynthesis isn't just about plant growth—it’s about minting the "carbon currency" required to hire a microbial workforce. The Exudate Menu: A …
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The Underground Economy: Carbon as Currency
Biological Gold: Why photosynthesis isn't just about plant growth—it’s about minting the "carbon currency" required to hire a microbial workforce.
The Exudate Menu: A breakdown of Monosaccharides (fast cash), Polysaccharides (savings accounts), and Organic Acids (specialized mining tools).
The Trading Post: How the plant uses targeted "buy orders" to trade energy for the specific minerals it needs.
The Rhizophagy Revolution
The "Commuter" Microbes: Understanding the research from Dr. James White’s lab at Rutgers.
The Microbial Car Wash: A technical look at how plants lure, strip, "milk," and eject bacteria to scavenge for nutrients.
Endophytes vs. Rhizophagy: Distinguishing between long-term "tenants" inside the plant and the transient "workers" in the root tip.
The Biomimicry Reality Check
Ecology vs. Agronomy: Why nature optimizes for survival, while growers optimize for yield, quality, and consistency.
The Managed System: Why an indoor grow or greenhouse is not a wilderness, and why treating it as such often leads to inefficiencies and "natural" bottlenecks.
The "Selfish" Microbe: Understanding Immobilization and Stoichiometry—why microbes sometimes "rob" your plants of nitrogen to build their own populations.
Precision Biology & Biosecurity
The Risk of Raw Inputs: Why compost can be the highest risk factor for heavy metals, PFAs, herbicide residues, and pathogens like Pythium.
The Specialist Shop: Utilizing lab-grown consortiums for a cleaner, scalable facility.
Mycorrhizal Fungi: The role of Rhizophagus irregularis in Phosphorus mining.
Nutrient Unlockers: Using high-CFU strains like Microbial Mass or Mammoth P for data-backed biomass increases.
Trichoderma: Beyond biocontrol—how it uses siderophores to "magnetize" insoluble iron and trigger Induced Systemic Resistance.
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Lean Farming: Running an Efficient Grow with Ben Hartman (Foundations Series #2)
This episode is part of the Foundations of Soil & Plant Science series — a selection of conversations that continue to influence how I approach soil health, plant performance, and cultivation systems. I’ve …
Shownotes
This episode is part of the Foundations of Soil & Plant Science series — a selection of conversations that continue to influence how I approach soil health, plant performance, and cultivation systems. I’ve added a short new intro to each re-release with updated perspective and context.
Originally released as Episode 20
My guest for this episode is Ben Hartman, farmer and author of The Lean Farm.
Ben operates Clay Bottom Farm in Goshen, Indiana, and is known for applying lean manufacturing principles to agriculture. His work focuses on improving farm efficiency, reducing wasted time and materials, and designing production systems that prioritize value and profitability.
In this conversation we discuss:
What lean farming means and how it applies to cultivation
Identifying and eliminating wasted time, labor, and inputs
Designing efficient workflows and production systems
How simplifying systems can improve consistency and profitability
Applying lean principles to indoor and greenhouse cultivation
While this conversation comes from outside traditional cannabis production, the principles discussed are directly applicable to any cultivation operation looking to improve efficiency and long-term sustainability.
Part of the Foundations of Soil & Plant Science series.
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Silicon for Plant Health and Stress Resistance with Dr. Wendy Zellner (Foundations Series #1)
My guest for this episode is Dr. Wendy Zellner.Dr. Zellner received her doctorate from the University of Toledo in 2012, where she trained in molecular and cellular biology with a focus on the role of silicon …
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My guest for this episode is Dr. Wendy Zellner.
Dr. Zellner received her doctorate from the University of Toledo in 2012, where she trained in molecular and cellular biology with a focus on the role of silicon in plant defense responses. She then completed a four-year postdoctoral position with the USDA-ARS, continuing her research on how silicon can alleviate both abiotic and biotic stress in crops such as tomato and tobacco.
Her work has included developing standard methods for quantifying silicon uptake from fertilizer materials, identifying uptake mechanisms for silicon in model species, and evaluating how those pathways function across a range of crop systems.
In this conversation we cover:
The role of silicon in plant physiology and structure
Silicon and plant defense against abiotic and biotic stress
How plants take up and utilize silicon
Misconceptions around silicon fertilizers and supplements
Practical considerations for growers using silicon inputs
This remains one of the most comprehensive discussions we’ve had on silicon and plant health, and it continues to be highly relevant for growers looking to better understand how this element functions within a complete fertility program.
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Beneficial Fungi for Cannabis IPM: Beauveria and Blastospores Explained with Julie Graesch
Guest: Julie GraeschBiological Scientist | IPM SpecialistCurrently with BioWorksFormerly: BASF, Becker Underwood, Iowa State UniversityTopics Covered:What “entomopathogenic fungi” actually meansHow fungi like …
Shownotes
Guest: Julie Graesch
Topics Covered:
Biological Scientist | IPM Specialist
Currently with BioWorks
Formerly: BASF, Becker Underwood, Iowa State UniversityWhat “entomopathogenic fungi” actually means
How fungi like Beauveria and Metarhizium infect insect pests
Differences between blastospores and conidia
Why formulation and environmental conditions matter
Common reasons fungal biocontrol applications fail
Preventative vs. reactive use of entomopathogenic fungi
Integrating fungal biologicals into a broader IPM strategy
Key Takeaways:Entomopathogenic fungi are biological tools, not chemical knockdowns
Understanding fungal life cycles improves consistency and outcomes
Blastospores behave differently than traditional spore formulations
Successful IPM relies on timing, environment, and integration—not rescue sprays
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Growing Cannabis from Seed: Is Seed-Based Cultivation the Future? with Dr. Matthew Indest
n this conversation with plant scientist and commercial cultivation strategist Dr. Matthew Indest, we explore the emerging frontier of seed-based cannabis production and what it would take for the industry to …
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n this conversation with plant scientist and commercial cultivation strategist Dr. Matthew Indest, we explore the emerging frontier of seed-based cannabis production and what it would take for the industry to move beyond clonal propagation.
We cover:
The agronomic upside of seed-grown plants (disease resistance, vigor, adaptability, cost savings)
The scientific and operational barriers preventing widespread seed adoption
How genetic stability, inbreeding depression, and trait selection differ between cannabis and traditional crops
Data-driven cultivation strategies for evaluating new seed lines
Whether seed-based production can truly rival clonal systems for consistency and top-tier flower quality
What the next decade of cannabis breeding and cultivation might look like
About Dr. Indest:
Matthew holds a Ph.D. in plant science and has spent more than a decade bridging academic research with applied cannabis production. He has led breeding and cultivation programs across indoor, greenhouse, and outdoor operations and focuses on developing high-performing cultivars and integrated, data-backed production systems.Hosted by Simplecast, an AdsWizz company. See pcm.adswizz.com for information about our collection and use of personal data for advertising.
Cannabis Growing Basics: pH, Water, Light and Nutrients with Bryant Mason
This wide-ranging conversation with Bryant Mason covers a series of essential—but frequently misunderstood—topics in organic cultivation. We explore why simple, low-tech data collection strategies often …
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This wide-ranging conversation with Bryant Mason covers a series of essential—but frequently misunderstood—topics in organic cultivation. We explore why simple, low-tech data collection strategies often outperform high-tech systems and how to interpret those readings in the real world. From there, we break down how pH truly behaves in biologically active soils, what your water test results do and don’t tell you, and how water chemistry shapes nutrient availability.
We also discuss practical considerations for under-canopy lighting, when it helps and when it’s a distraction, and dig into the recurring challenges Bryant sees around phosphorus and nitrogen management in living soil. Throughout the episode we jump between concepts, case studies, and troubleshooting insights that growers can apply immediately.
Topics Covered:
Low-tech monitoring and data-collection approaches
pH dynamics in organic systems
Water testing and interpreting results
Under-canopy lighting: pros, cons, and real-world use
Phosphorus and nitrogen management in living soil
Practical troubleshooting and decision-making strategies
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Grow Light Spectrum for Cannabis: A Practical Guide with Dr. David Hawley of Fluence (Part 2)
In this conversation, Tad and Dr. David Hawley (Chief Science Officer at Fluence) unpack what really matters when dialing in your lighting strategy for cannabis and other high-value crops. They get deep into …
Shownotes
In this conversation, Tad and Dr. David Hawley (Chief Science Officer at Fluence) unpack what really matters when dialing in your lighting strategy for cannabis and other high-value crops. They get deep into how plants perceive light, what “good spectrum” actually means, and how to think about intensity, uniformity, and plant response in a more scientific (and practical) way.
Topics covered include:
Understanding Daily Light Integral (DLI) and how it relates to plant metabolism
When to use far-red and what to expect from it
The truth about UV lighting and secondary metabolite production
Managing leaf temperature and avoiding hidden stress from high PPFD
How fixture placement and uniformity impact yield and morphology
Using dimming strategies through different growth stages
Why overemphasizing spectrum specs can distract from the real gains
Practical steps for mapping your grow and verifying performance
David also shares insights on under-canopy lighting, canopy architecture, and the future of horticultural light design, offering takeaways for both commercial cultivators and serious home growers.
Resources mentioned:
Fluence by OSRAM
KiS Organics Living Soil & Nutrients
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Grow Light Spectrum for Cannabis: A Practical Guide with Dr. David Hawley of Fluence (Part 1)
In this conversation, Tad and Dr. David Hawley (Chief Science Officer at Fluence) unpack what really matters when dialing in your lighting strategy for cannabis and other high-value crops. They get deep into …
Shownotes
In this conversation, Tad and Dr. David Hawley (Chief Science Officer at Fluence) unpack what really matters when dialing in your lighting strategy for cannabis and other high-value crops. They get deep into how plants perceive light, what “good spectrum” actually means, and how to think about intensity, uniformity, and plant response in a more scientific (and practical) way.
Topics covered include:
Understanding Daily Light Integral (DLI) and how it relates to plant metabolism
When to use far-red and what to expect from it
The truth about UV lighting and secondary metabolite production
Managing leaf temperature and avoiding hidden stress from high PPFD
How fixture placement and uniformity impact yield and morphology
Using dimming strategies through different growth stages
Why overemphasizing spectrum specs can distract from the real gains
Practical steps for mapping your grow and verifying performance
David also shares insights on under-canopy lighting, canopy architecture, and the future of horticultural light design, offering takeaways for both commercial cultivators and serious home growers.
Resources mentioned:
Fluence by OSRAM
KiS Organics Living Soil & Nutrients
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Can Stress Improve Cannabis Quality? Secondary Metabolites with Dr. José Leme
Can stress actually improve flower quality? In this episode, I sit down with Dr. José Leme to dive into the science of plant stimuli and how growers can apply it in practice. We cover:🌿 Mechanical cues — how …
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Can stress actually improve flower quality? In this episode, I sit down with Dr. José Leme to dive into the science of plant stimuli and how growers can apply it in practice. We cover:
🌿 Mechanical cues — how super cropping and vibration trigger plant defense pathways.
💧 Water restriction — why moderate, timed drought can shift carbon toward secondary metabolites like terpenes and cannabinoids.
🧪 Jasmonates (MeJA) — when they increase trichome density, and what trade-offs you need to consider.
🔬 Trichome measurement pitfalls — why bracts are the gold standard for consistent data.
🌱 Propagation & trial design — the importance of clonal fidelity and standardized protocols when testing stress strategies.
Dr. Leme and I also highlight practical guardrails for growers: cultivar differences, timing, and the “sweet spot” between beneficial stress and damaging stress.
📄 Referenced Studies:
Water Deficit Stress and Secondary Metabolites
Jasmonates in Growth, Trichomes, and Cannabinoids
Metabolic Responses to Water Deficit
Trichome Sampling & Quantification Methods
Trigonelline as a Biomarker for Drought Stress (Preprint)
Propagation & Clonal Fidelity in Cannabis Research
Additional vibration study discussed in the episode.
🎧 Whether you’re a grower, consultant, or researcher, this conversation will give you a framework for using stress not as a risk—but as a precision tool to enhance plant quality.
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Cannabis Clone Propagation at Scale: 15,000 Clones a Year with Connor Ockfen
📝 Episode Highlights:Connor’s journey from greenhouse grower to propagation leaderScaling propagation: key infrastructure and protocolsHormonal treatments and substrate selection for optimal rootingManaging …
Shownotes
📝 Episode Highlights:Connor’s journey from greenhouse grower to propagation leader
Scaling propagation: key infrastructure and protocols
Hormonal treatments and substrate selection for optimal rooting
Managing cultivar libraries and mother plant health
IPM and environmental control strategies tailored for veg
Writing and refining SOPs for compliance and consistency
Track-and-trace integration in a regulated cannabis market
The role of living soil vs. hydroponics in Connor’s career
🧪 Mentioned in This Episode:Collective Elevation: www.collectiveelevation.com
KIS Organics Products & Podcast: www.kisorganics.com
Cannabis Cultivation & Science Conference: September 5–7, 2025, Bozeman, Montana — featuring Suzanne Wainwright-Evans, Dr. Greenhouse, Jeremy Plumb, and more. Early bird tickets available now!
Join our Patreon for bonus content: Patreon.com/KISOrganics
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How Plant Stress Shapes Cannabis Chemistry with Dr. Michael Gutensohn
In this episode of the Cannabis Cultivation and Science Podcast, we’re joined by Dr. Michael Gutensohn, an expert in plant physiology and secondary metabolism, to explore the intricate relationship between …
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In this episode of the Cannabis Cultivation and Science Podcast, we’re joined by Dr. Michael Gutensohn, an expert in plant physiology and secondary metabolism, to explore the intricate relationship between environmental stress and the production of secondary metabolites in cannabis. We discuss how various abiotic stressors—such as light intensity, temperature fluctuations, and nutrient availability—can influence the biosynthesis of key phytochemicals, including cannabinoids, terpenes, and flavonoids. Dr. Gutensohn shares insights from his research on plant defense mechanisms, metabolic signaling pathways, and how growers might harness controlled stress to enhance both plant resilience and phytochemical output. Whether you’re a cultivator aiming to fine-tune your grow environment or a scientist delving into cannabis metabolomics, this episode offers valuable, research-backed perspectives on optimizing secondary metabolite production through stress modulation. Hosted by Simplecast, an AdsWizz company. See https://pcm.adswizz.com for information about our collection and use of personal data for advertising.Logan Labs Soil Testing and Nitrogen Management for Cannabis with Bryant Mason
Welcome to the Cannabis Cultivation and Science podcast, I'm your host Tad Hussey of KIS Organics. This is the podcast where we discuss the cutting edge of growing from a science based perspective and draw on …
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Welcome to the Cannabis Cultivation and Science podcast, I'm your host Tad Hussey of KIS Organics. This is the podcast where we discuss the cutting edge of growing from a science based perspective and draw on top experts from around the industry to share their wisdom and knowledge. My guest this week is Bryant Mason. I’ve had Bryant on the podcast a few times in the past and he’s one of my favorite guests.
Bryant is a Certified Crop Advisor who specializes in organic cannabis nutrition. He has spent the last 8 years working to combine precision laboratory techniques with data science in an effort to unravel the complexities of cannabis soil and crop nutrition. Along with his work on soilless media, he also helps outdoor cultivators around the U.S. with nutrient management and cover cropping. If you would like to do soil testing, help interpreting a soil test, or are interested in an online course on these subjects, his website is www.soildoctorconsulting.com. If you want to save $100 on his course, you can use the discount code KISORGANICS, all one word. I’ve taken his course and was recently going back to review some of the content. It’s excellent and I highly recommend it for anyone wanting to do a deep dive on cannabis fertility and re-using your soil.
After recording the podcast with Bryant, he had a couple of points he wanted to clarify so there is a short addendum that I added in at the very beginning of the podcast.
Now on to the show!
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Growing Cannabis in a Greenhouse: Practical Management Advice with Jared Ferguson
Before starting the podcast, I wanted to share about an exciting new conference that we are hosting in conjunction with Collective Elevation in Bozeman, Montana. I’ve been talking to Suzanne Wainwright Evans …
Shownotes
Before starting the podcast, I wanted to share about an exciting new conference that we are hosting in conjunction with Collective Elevation in Bozeman, Montana. I’ve been talking to Suzanne Wainwright Evans about hosting a conference for years, and Adam at Collective graciously offered to open up his doors and let us host it on his property. The event is happening on September 5-7th, 2025 and I’m super excited. We are kicking it off with workshops from The Bug Lady Suzanne and Dr. Greenhouse, followed by a facility tour and then a bunch of excellent speakers and networking events over the next 2 days. Then on the 7th, the Montana Living Soil Alliance is hosting a tasting event that will be included in the price of the conference ticket. The tasting event will be a private event, with cannabis donated by the Living Soil Alliance, as a way to have an amazing sensory experience and see what premium quality Montana Living Soil has to offer. Tickets are limited to 200 spots, so be sure to grab a ticket if you’re interested. Tickets are now on sale at kisorganics.com and the conference is called the Cannabis Cultivation and Science Conference.
In honor of the conference, I wanted to do an interview series with some of the amazing folks at Collective Elevation to give a growers perspective and share some of what they’ve been able to accomplish. I’ve already had Adam Arnold on the podcast and you can also view a tour of their facility on the kisorganics youtube page.
My guest this week is Jared Ferguson, the Greenhouse Manager at Collective Elevation. He’s been working at Collective for over 4 years now, but prior to that he studied Environmental horticulture at Montama State University in bozeman. During which he worked for the universities greenhouse facility and research farm, where hje got his first experiences in mixing soil, amending plants, managing irrigation, and conducting IPM processes. Now on to the show!
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Cannabis Pest Management Tips with Saul Alba
Saul began his agriculture work in vineyard and winery operations on the Central California coast. After deciding to focus his career on crop protection he moved north where he managed IPM programs at Monterey …
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Saul began his agriculture work in vineyard and winery operations on the Central California coast. After deciding to focus his career on crop protection he moved north where he managed IPM programs at Monterey Mushroom Company and Jacobs Farm/Del Cabo. This work led to a position as IPM Manager at Harborside Farms where he directed their bio-intensive greenhouse cannabis IPM program. Saul now works as a Technical Sales Representative for BioBee USA.
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HVAC and Environmental Controls for Cannabis Grows with Dr. Nadia Sabeh (Dr. Greenhouse)
Dr. Nadia Sabeh is the President and Founder of Dr. Greenhouse, Inc., an agricultural and mechanical engineering firm that specializes in the design of HVAC systems for indoor plant environments. Dr. Sabeh has …
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Dr. Nadia Sabeh is the President and Founder of Dr. Greenhouse, Inc., an agricultural and mechanical engineering firm that specializes in the design of HVAC systems for indoor plant environments. Dr. Sabeh has her PhD in Agricultural Engineering from the University of Arizona’s Controlled Environment Agriculture Center (CEAC) and is a licensed Mechanical Engineer in the State of California. For over 20 years, she has dedicated her education and career to helping farmers control their environments, allowing them to grow crops indoors, in greenhouses and in facilities that would otherwise be impossible or impractical to do so.
Nadia has a free VPD app for both iPhone and Android as well as an amazing podcast called "The Doctor is in" that's available on all streaming platforms. https://www.doctorgreenhouse.com/podcast/tag/podcast
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