Skip to content

252: Acid-Fast Bacilli (AFB): An Introduction

November 13, 2026 · 36 min

Show notes

It's time to revisit acid-fast bacilli (AFB)!

What is acid-fastness? What stains are used to detect AFB, and how are AFB cultures processed?

In this episode of Let's Talk Micro, Luis revisits a conversation with Dr. Filipe Muniz (Cerqueira) about the fundamentals of AFB. They discuss acid-fast staining, specimen processing, culture media, and the Runyon groups. Dr. Muniz also discusses nontuberculous mycobacteria (NTM), including examples of clinically relevant organisms and their characteristics.

Whether you're new to clinical microbiology or looking for a refresher, tune in to this episode from the archives!

Note: This episode was originally released on January 30TH 2025. At the time of recording, Dr. Muniz was serving as a clinical microbiology laboratory director at the University of Alabama at Birmingham (UAB). He is no longer in that role and is currently a medical student at UAB.

Stay connected with Let's Talk Micro:

Support the podcast:

All episodes of Let’s Talk Micro

Settings

Display

Language

Decides which stations you see first on the home page.

History

Recently played stations and episodes and your latest searches are only stored in this browser.

Notifications

A short notice when a podcast you follow has a new episode. Turn off single podcasts with the bell on their page.

Account

Save favorites – in the app and on the website.

Keyboard shortcuts

/
Open search
Space
Play or pause
← →
In the player: back 15 s, forward 30 s (episodes) · in a row: next tile
↑ ↓
In a row: target within the tile
Home / End
In a row: first or last tile
Esc
Close window or menu
?
This overview
Now playing

Go to station page

Play next

    Add episodes with “Play next” – they play after the current episode.